Bartušková earned her bachelor’s degree in management and economics from the College of Business in 2010.
In 2004–2007, she worked as office manager for the Ateliér Petrov architecture studio and in 2007–2009 she was head of sales and production administration at Eastmedia, s. r. o.

She joined the Gallery of the Central Bohemian Region in 2009 and currently holds the position of Head of PR & Marketing, where she is responsible for communications with the public and the media and for the gallery’s marketing activities.